Question 1

Which of the following can be examined with an ordinary bright-field microscope?

Accepted Answer

A)   wet mounts
B)   heat-fixed specimens
C)   chemical-fixed specimens
D)   all of the choicesE) B) and C)
F) A) and D)

Question 2

Ten percent Calcofluor White stain is often used in wet mounts of

Accepted Answer

A)   bacteria.
B)   parasites.
C)   molds.
D)   protozoa.E) A) and B)
F) A) and C)

Question 3

Viral replication in cell cultures can be detected by

Accepted Answer

A)   the observation of cytopathic effects.
B)   hemadsorption.
C)   gram stain.
D)   the observation of cytopathic effects and hemadsorption.E) B) and C)
F) C) and D)

Question 4

An elevated IgM titer typically indicates an active infection while an elevated IgG titer does not distinguish between active and past infections.

Accepted Answer

A) True 
 B)False

Question 5

Standard microbiological practices include all of the following except

Accepted Answer

A)   Work space should be disinfected only when contaminating spills occur.
B)   Eating, drinking, manipulation of contact lenses, and the use of cosmetics, gum, and tobacco products are strictly prohibited in the lab.
C)   Lab personnel should know how to use the emergency eyewash and shower stations.
D)   Hair longer than shoulder length should be tied back.E) C) and D)
F) B) and D)

Question 6

Which of the following is useful in the identification of bacteria?

Accepted Answer

A)   source of the culture specimen
B)   growth patterns on selective and differential media
C)   hemolytic, metabolic, and fermentative properties
D)   all of the choicesE) A) and B)
F) A) and C)

Question 7

If one is looking for intracellular inclusion bodies, one would suspect the microorganism to be a

Accepted Answer

A)   mycoplasma.
B)   rickettsia.
C)   chlamydia.
D)   protozoan.E) None of the above
F) B) and C) C

Question 8

The indirect immunosorbent assay detects antibodies rather than antigens.

Accepted Answer

A) True 
 B)False

Question 9

Indirect immunofluorescence involves fixing the specimen onto a slide, adding fluorochrome-labeled antibodies and then incubating before examining on the microscope.

Accepted Answer

A) True 
 B)False

Question 10

Some viruses alter the plasma membrane of infected cells in such a way as to cause red blood cells to tightly adhere to the infected cells. This is called ___________.

Accepted Answer

hemadsorption

Question 11

Recommended guidelines for laboratory microbiological precautions should reflect the laboratory's ________ _________.

Accepted Answer

biosafety level

Question 12

Fungal infections are often diagnosed by direct microscopic examination of specimens.

Accepted Answer

A) True 
 B)False

Question 13

Which of the following tests can be used to distinguish Escherichia from Enterobacter?

Accepted Answer

A)   IMVIC
B)   ONPG test
C)   citrate utilization
D)   starch hydrolysisE) All of the above
F) B) and D)

Question 14

Which of the following tests is an agglutination test for the bacterium causing typhoid fever?

Accepted Answer

A)   Quellung reaction
B)   API 20E system
C)   Widal test
D)   IMViCE) B) and C)
F) A) and B)

Question 15

The identification of mycoplasmas in clinical specimens can be quickly done because of their rapid growth rate in culture.

Accepted Answer

A) True 
 B)False

Question 16

Which of the following is routinely used to detect spirochetes in skin lesions in early syphilis?

Accepted Answer

A)   bright-field microscopy
B)   phase-contrast microscopy
C)   dark-field microscopy
D)   DNA fingerprintingE) A) and D)
F) A) and C)

Question 17

Detection of single or multiple microorganisms based on their cell size is known as

Accepted Answer

A)   flow cytometry.
B)   complement fixation.
C)   agglutination.
D)   serotyping.E) A) and B)
F) A) and C)

Question 18

Which of the following media will permit the growth of one group of bacteria while inhibiting the growth of some other groups?

Accepted Answer

A)   differential
B)   selective
C)   enrichment
D)   characteristicE) A) and D)
F) None of the above

Question 19

Which of the following uses a radioisotope?

Accepted Answer

A)   RIA
B)   ELISA
C)   API 20E
D)   all of theseE) None of the above
F) B) and C)

Question 20

Which of the following should be isolated only by specialized research laboratories?

Accepted Answer

A)   mycoplasmas
B)   rickettsias
C)   chlamydiae
D)   all of the choicesE) B) and C)
F) C) and D)

Exam 35: Clinical Microbiology and Immunology

Which of the following can be examined with an ordinary bright-field microscope?

Correct AnswerverifiedShow Answer

Ten percent Calcofluor White stain is often used in wet mounts of

Correct AnswerverifiedShow Answer

Viral replication in cell cultures can be detected by

Correct AnswerverifiedShow Answer

An elevated IgM titer typically indicates an active infection while an elevated IgG titer does not distinguish between active and past infections.

Correct AnswerverifiedShow Answer

Standard microbiological practices include all of the following except

Correct AnswerverifiedShow Answer

Which of the following is useful in the identification of bacteria?

Correct AnswerverifiedShow Answer

If one is looking for intracellular inclusion bodies, one would suspect the microorganism to be a

Correct AnswerverifiedC

The indirect immunosorbent assay detects antibodies rather than antigens.

Correct AnswerverifiedShow Answer

Indirect immunofluorescence involves fixing the specimen onto a slide, adding fluorochrome-labeled antibodies and then incubating before examining on the microscope.

Correct AnswerverifiedShow Answer

Some viruses alter the plasma membrane of infected cells in such a way as to cause red blood cells to tightly adhere to the infected cells. This is called ___________.

Correct Answerverifiedhemadsorption

Recommended guidelines for laboratory microbiological precautions should reflect the laboratory's ________ _________.

Correct Answerverifiedbiosafety level

Fungal infections are often diagnosed by direct microscopic examination of specimens.

Correct AnswerverifiedShow Answer

Which of the following tests can be used to distinguish Escherichia from Enterobacter?

Correct AnswerverifiedShow Answer

Which of the following tests is an agglutination test for the bacterium causing typhoid fever?

Correct AnswerverifiedShow Answer

The identification of mycoplasmas in clinical specimens can be quickly done because of their rapid growth rate in culture.

Correct AnswerverifiedShow Answer

Which of the following is routinely used to detect spirochetes in skin lesions in early syphilis?

Correct AnswerverifiedShow Answer

Detection of single or multiple microorganisms based on their cell size is known as

Correct AnswerverifiedShow Answer

Which of the following media will permit the growth of one group of bacteria while inhibiting the growth of some other groups?

Correct AnswerverifiedShow Answer

Which of the following uses a radioisotope?

Correct AnswerverifiedShow Answer

Which of the following should be isolated only by specialized research laboratories?

Correct AnswerverifiedShow Answer

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C

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hemadsorption

Recommended guidelines for laboratory microbiological precautions should reflect the laboratory's _.

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biosafety level

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